Journal article
Establishment of single-cell transcriptional states during seed germination
LC Liew, Y You, L Auroux, M Oliva, M Peirats-Llobet, S Ng, M Tamiru-Oli, O Berkowitz, UVT Hong, A Haslem, T Stuart, ME Ritchie, GW Bassel, R Lister, J Whelan, Q Gouil, MG Lewsey
Nature Plants | NATURE PORTFOLIO | Published : 2024
Abstract
Germination involves highly dynamic transcriptional programs as the cells of seeds reactivate and express the functions necessary for establishment in the environment. Individual cell types have distinct roles within the embryo, so must therefore have cell type-specific gene expression and gene regulatory networks. We can better understand how the functions of different cell types are established and contribute to the embryo by determining how cell type-specific transcription begins and changes through germination. Here we describe a temporal analysis of the germinating Arabidopsis thaliana embryo at single-cell resolution. We define the highly dynamic cell type-specific patterns of gene exp..
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Grants
Awarded by La Trobe University
Funding Acknowledgements
Work in M.G.L.'s laboratory is funded by the Australian Research Council (ARC) Discovery Program (grant DP220102840). M.G.L. and R.L. are funded by ARC CE230100015. Q.G. is funded by a National Health and Medical Research Council Investigator Grant (GNT2007996). J.W. and L.C.L. were funded by the ARC Discovery Program (grant DP210103258). This study was supported by the La Trobe University Genomics Platform. This work was also supported by National Health and Medical Research Council Investigator Grant GNT1178460 and ARC grants DP210104058, LE170100225 and CE140100008 (all to R.L.). L.A. was supported by a La Trobe University Graduate Research Scholarship and a La Trobe University Full Fee Research Scholarship. We thank B. Wakely and O. Nath for expertise and assistance with building the interactive web browser.